The increasing accumulation of plastics in the environment has raised concerns regarding their potential health hazards. Nanoplastics (NPs) can get transported across the placental barrier, resulting in detrimental effects on developing offspring. To date, the effects of maternal exposure to NPs during pregnancy on the cardiac toxicity in adult offspring have not been conclusively evaluated. Herein, the potential for cardiac injury in the progeny of adult mice that were gestationally exposed to 80 nm polystyrene NPs (PS-NPs) at different doses (0, 0.5, 1, and 5 µg µL