Nghiên cứu tạo chủng virus gây hội chưng rối loạn hô hấp và sinh sản nhược độc từ chủng cường độc=Study on attenuated Porcine reproductive and respiratory syndrome virus from highly pathogenic virus strain

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Tác giả: Hiên Nguyễn Bá, Nguyễn Thị Hoa, Lan Nguyễn Thị, Mỹ Lệ Huỳnh Thi, Nam Nguyễn Hữu, Phạm Hồng Ngân, Phạm Ngọc Thạch, Nguyễn Văn Thanh, Phương Lê Huỳnh Thanh, Thâu Trịnh Đình, Nguyễn Văn Thọ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2018

Mô tả vật lý: tr.5

Bộ sưu tập: Metadata

ID: 545992

Thêm vào giỏ

Chủng virus gây Hội chứng rối loạn hô hấp và sinh sản nhược độc được tạo ra bằng việc cấy chuyển liêntiếp 90 đời chủng virus cường độc KTY-PRRS-06 trên môi trường tế bào Marc145. Đặc tính nhân lên của virus,hiệu giá virus, đặc điểm di truyền của gen ORF5, ORF7, NSP2 được xác định ở mỗi 10 đời cấy chuyển. Độclực của virus ở đời cấy chuyển 1 và 90 được kiểm tra trên lợn thí nghiệm. Kết quả nghiên cứu đã chỉ ra rằngchủng virus ở đời cấy chuyển thứ 90 gây bệnh tích tế bào sau 12h gây nhiễm và hủy hoại toàn bộ tế bào sau 48hgây nhiễm. Hiệu giá virus, trình tự gen ORF5, ORF7, NSP2 được giữ ổn định ở đời cấy chuyển thứ 50 đến đờicấy chuyển 90. Chủng virus PRRS nhược độc được tạo ra khi tiêm cho lợn thí nghiệm không gây bệnh cho lợnvà có khả năng kích thích cơ quan miễn dịch của lợn sản sinh kháng thể đặc hiệu kháng PRRSV.The attenuated porcine reproductive and respiratory syndrome virus was created from 90continuously passage inoculation generations of the highly pathogenic KTY-PRRS-06 virus strainon the Marc145 cell medium. The virus multiplied feature, viral titre, genetic characteristics of ORF5,ORF7, NSP2 genes were determined in each of 10 passages. The piglets were inoculated with theKTY-PRRS-06 virus at the 1st passages and 90th passages to test virulence of virus. The studiedresults showed that the virus strain at the 90th passages exhibited cytopathogenic effect after 12hours of infection and the cells were completely destroyed after 48 hours of infection. Viral titre,ORF5, ORF7, NSP2 gene sequences were maintained stably from the 50th to 90th passages. Thisattenuated virus strain did not cause disease in the experimental pigs and it h

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