Evaluate of iron condition by using concentrated quantification test for ferric, ferritin, serum transferrin and transferrin saturation that is necessary for treatment of iron deficiency anemia in hemodialysis (HD) patients. Purpose: study on iron concentration, ferritin, transferrin, calculation of serum saturation transferrin and iron condition of chronic kidney patients on chronic hemodialysis following 2006 K/DOQI guidelines. Method: Concentration measurement of iron, ferritin, transferrin, serum saturation transferrin and rate of iron deficiency of 70 chronic kidney patients on chronic hemodialysis. Results: iron concentration: normal in 50 patients (71,4 percent), increased in 17 patients (24,3 percent), and decreased in 3 patients (4,3 percent). Ferritin concentration: normal in 7 patients (10 percent), increased in 63 patients (90 percent), no patients with decreased ferritin concentration. Transferrin concentration: normal in 6 patients (8,6 percent), no patients with increased transferrin, decreased in 64 patients (91,4 percent). TSAT: normal in 24 patients (33,4 percent), increased in 38 patients (54,2 percent), decreased in 8 patients (11,4 percent). 36 (51,5 percent) of the patients had iron overload. Conclusion: Majority of patients have regular serum iron concentration, increased ferritin and decreased transferrin concentration. Over a half of patients were in overload of iron condition.