Dịch tễ phức tạp của bệnh sán lá gan nhỏ gây bởi loại Opisthorchis viverrnini tại miền trung Việt Nam do xuất hiện thêm cận loài Opisthorchis sp BD2013 gây bệnh trên vịt=Complicated epidemiology of small liver tape-worm disease caused by Opisthorchis viverrini species in central area, Viet Nam due to occurrence of Opisthorchis sp. BD2013 species caused disease in duck

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Bích Thủy Nguyễn Thị, Dorny Pierre, Gabriel Sarah, Thanh Nguyễn Thị Giang, Lê Thanh Hòa, Hà Đào Thị Thanh

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2018

Mô tả vật lý: tr.53

Bộ sưu tập: Metadata

ID: 547837

Thêm vào giỏ

Chúng tôi đã tiến hành nghiên cứu điều tra cắt ngang tại vùng dịch tễ tỉnh Bình Định để xác định tỷ lệ nhiễmvà các yếu tố nguy cơ của bệnh sán lá gan nhỏ trên cả vật chủ cuối cùng là ngừơi và vịt, và vật chủ trung gianlà ốc và cá nước ngọt, đồng thời chúng tôi tiến hành giải trình tự gien ty thể của loài sán lá gan được tìm thấytrên vịt để xác định vị trí di truyền học của loài sán này trên cây phả hệ của họ Opisthorchiidae trong mối quanhệ với các loài Opisthorchis trong họ đã được tìm thấy trước đó. Kết quả nghiên cứu cho thấy tỷ lệ nhiễm bệnhsán lá gan nhỏ (O. viverrini) trong cộng đồng dân cư được điều tra tại tỉnh Bình Định là 11,4 % và tỷ lệ nhiễmbệnh sán lá gan nhỏ (Opisthorchis sp BD 2013) trên vịt là 34,3 %. Hai loài ốc nước ngọt Bithynia siamensisgoniomphalos và B. funiculata (Bithyniidae) được xác định là vật chủ trung gian thứ nhất của sán lá gan nhỏ O.viverrini tại Việt Nam và 10 loài cá nước ngọt, chủ yếu thuộc họ Cyrinidae được xác định là vật chủ trung gianthứ 2, trong đó cá diếc Carassius auratus được dùng để làm gỏi cá có tỷ lệ nhiễm ấu trùng sán lá gan nhỏ O.viverrini cao nhất. Kết quả nghiên cứu cũng chỉ ra rằng, ấu trùng của cả hai loài sán lá gan nhỏ O. viverrini vàOpisthorchis sp BD2013 được tìm thấy trên cùng một vật chủ trung gian thứ nhất là ốc Bithynia funiculata vàtrên cùng vật chủ trung gian thứ 2 là 3 loài cá nước ngọt có tên Esomus metallicus, Puntius brevis và Rasboraaurotaenia. Kết qủa phân tích hệ gien cho thấy, trên cây phả hệ của hệ gien ty thể (nad1, cob và cox1) Opisthorchis sp BD2013 thể hiện là một loài độc lập và có quan hệ cận loài với O. viverrini. Mặc dù kết quả nghiên cứuhiện tại chưa tìm thấy Opisthorchis sp BD2013 trên người, song việc đồng thời lưu hành hai loài sán lá gan cóquan hệ cận loài và chia sẻ cùng vật chủ trung gian ở giai đoạn ấu trùng gây bệnh trong cùng vùng dịch tễ, cónguy cơ làm phức tạp dịch tễ học của bệnh bởi việc giao phối chéo hay chuyển gien có thể xảy ra khi vật chủcuối cùng nhiễm cùng lúc cả 2 loài sán lá gan có quan hệ cận loài này.The cross sectional studies at the epidemiological area in Binh Dinh province was conducted todefine the prevalence and risk factors of the small liver tape-worm disease caused by O. viverrinispecies in both last hosts, such as: human and duck, and the intermediate hosts, such as: snailand fish. In addition, the gene sequence in mitochondrial of the identified small liver tape-wormswas decoded in order to define the genetic position of this small liver tape-worm species in thephylogenetic tree of the Opisthorchiidae family in relationship with the Opisthorchis species in thisfamily that was identified prior. The studied result showed that the infection rate of human with O.viverrini in the surveyed communities in Binh Dinh province was 11.4% and the infection rate ofduck with Opisthorchis sp BD 2013 was 34.3%. The two snail species, such as: Bithynia siamensis goniomphalos and B. funiculata were determined to be the first intermediate hosts and 10 fresh waterfish species, belonging to Cyprinidae family were determined to be the second intermediate hosts ofthe small liver tape-worm (O. viverrini) in Viet Nam. Of which, the infection rate of Carassius auratusspecies with (the raw meat of this fish species was often eaten by the communities in the surveyedregion) O. viverrini was highest. Also, the studied result indicated that O. viverrini and Opisthorchis spBD2013 were identified in the same 1st intermediate host (snail species: Bithynia funiculata) and 2ndand 3rd intermediate hosts (fish species: Esomus metallicus, Puntius brevis and Rasbora aurotaenia).The result of analysing genes (nad1, cob and cox1) in mitochondrial and phylogenetic tree indicatedthat Opisthorchis sp BD2013 was an independent small tape-worm species, it related closely to O.viverrini. Although, the Opisthorchis sp BD2013 species was not found in human, but the sympatricdistribution and sharing the same intermediate hosts in the same epidemiological area of these smallliver tape-worm species indicated that the risk of creating a complicated epidemiology of the smallliver tape-worm infection due to crossing and gene transfer between two closely relation small livertape-worm species may occur in the same last host.

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR