Đa dạng gen 18S RRNA của trùng lông Balantidium coli gây tiêu chảy trên heo sau cai sữa ở một số tỉnh phía Nam=Diversification of 18S rRNA gene of Balatidium coli caused diarrhea in post-weaning piglets in some Southern provinces Do Tien Duy, Nguyen Le Dinh Phuong, Le Vo Truong

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Tác giả: Phương Nguyễn Lê Đình, Đỗ Tiến Duy, Nguyễn Phạm Huỳnh, Nguyễn Tất Toàn, Duy Lê Võ Trường

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2018

Mô tả vật lý: tr.43

Bộ sưu tập: Báo, Tạp chí

ID: 547857

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Mục tiêu của nghiên cứu này nhằm xác định sự đa dạng kiểu gen 18S rRNA của trùng lông Balantidiumcoli gây tiêu chảy trên heo sau cai sữa ở các trại heo của một số tỉnh phía Nam. Hai mươi chủng B. coli đượcphân lập từ 20 con heo sau cai sữa từ 30 đến 45 ngày tuổi có triệu chứng tiêu chảy đặc trưng. Giải trình tựgen của những chủng vi khuẩn này đã được phân tích và so sánh. Kết quả nghiên cứu cho thấy mức tươngđồng chuỗi nucleotide của các chủng B. coli trong nghiên cứu này là từ 97,4% đến 99,6%. Sự đột biến trênđoạn gen gồm các kiểu thay thế, mất đi hay chèn thêm một hay nhiều nucleotide so với các chủng tham chiếu(SP08 - Tây Ban Nha). Trên cây phát sinh loài, các chủng B. coli trong nghiên cứu này được chia thành 2nhóm (nhóm A gồm các chủng nằm cùng nhánh với các trình tự từ Nhật Bản, Trung Quốc và nhóm B gồmcác chủng nằm cùng nhánh với các trình tự từ Tây Ban Nha và Philippines). Đặc biệt, chủng B. coli thu thậpở tỉnh Đồng Nai và Bến Tre gồm các trình tự nằm ở cả hai nhóm trên cây phát sinh loài.The objective of this study aimed at determining the diversification of 18S rRNA gene ofBalantidium coli caused diarrhea in the post-weaning piglets in the pig breeding farms of somesouthern provinces. There were 20 B.coli strains isolated from 20 post-weaning piglets at 30-45days old having typical diarrhea. Gene sequences of these bacteria strains were analysed andcompared. The studied result showed that similarity level of nucleotide sequences of the B.colistrains in this study was 97.4 – 99.6%. The mutation in the gene segment included the replacetypes, loss and inserting one or more than one nucleotides in comparison with the referent bacteriastrains (SP08-Spain). The result of analyzing phylogenetic tree indicated that the B.coli strains inthis study were divided into 2 groups (group A including the strains located in the same branch withthe Chinese, Japanese strains and group B consisting of the strains located in the same branchwith the Philippines and Spain strains). Especially, the B.coli strains collecting from Dong Nai andBen Tre province located in both group A and B

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