Maize Dek570-1 affects the expression and function of organellar genes by performing cytidines-to-uridines RNA editing at specific sites of mitochondrial and chloroplast transcripts, thereby regulating seed and plant development. Cytidines-to-uridines (C-to-U) RNA editing at specific sites of mitochondrial and plastid transcripts is crucial for the expression and function of organellar genes, which requires PPR proteins. Here, we report the map-based cloning and characterization of Defective Kernel 570-1 (Dek570-1), which encodes a PPR-DYW protein and is an allele of Emp17. However, compared to the empty pericarp and embryonic lethality of emp17 (W22 background), dek570-1 (Zheng58 background) can produce small but viable seeds despite reducing the size of embryo and endosperm. dek570-1 plants are short and yellowed, but they can reproduce offspring. In mitochondria, loss-of-function of Dek570-1 abolishes the C-to-U editing at nad2-677 and ccmF