Microorganisms can produce various amphiphilic compounds known as biosurfactants, with diverse applications in distinct industries. This study was focused on the biosurfactant production by Limosilactobacillus fermentum HBUAS62516 for the synthesis of silver nanoparticles. The biosurfactant obtained was characterized as glycolipid using FTIR which showed prominent peaks at 2932.3, 1116.3, and 1084.4 cm