Staphylococcal SplA and SplB serine proteases target ubiquitin(-like) specific proteases.

• Tác giả: Uwe T Bornscheuer, Dominique Böttcher, Barbara M Bröker, Clemens Cammann, Felix L Glinka, Christian Hentschker, Elke Krüger, Michael Lammers, Michael Naumann, Ole Schmöker, Ulrike Seifert, Abhishek K Singh, Leif Steil, Uwe Völker
• Ngôn ngữ: eng
• Ký hiệu phân loại: 511.422 Splines
• Thông tin xuất bản: Germany : AMB Express , 2025
• Bộ sưu tập: NCBI
• ID: 549535

Tóm tắt

Staphylococcus aureus is a Gram-positive opportunistic pathogen that has colonized nearly 30% of the human population and can cause life-threatening infections. S. aureus exports a variety of virulence factors, such as a novel set of extracellular serine protease-like proteins (Spls). Spls are expressed by most clinical isolates of S. aureus, but their pathophysiological substrates and role during the infection are largely unknown. Here we characterized the substrate and cleavage specificity of recombinantly expressed SplA and SplB proteins. We identified a group of ubiquitin or ubiquitin-like modifying enzymes including deubiquitinating enzymes from human as well as from bacterial sources to be so far unknown SplA and SplB substrates. Distinct cleavage sites within these substrates for SplA (YLY