Two alkaloids, neotuberostemonine and bisdehydrotuberostemonine, and two non-alkaloidal metabolites, methyl trans-4-hydroxycinnamate and 5-(hydroxymethyl)furfural, were isolated from the roots of Stemona tuberosa plant, collected in Buon Don commune (Dak Lak province). Their structures were elucidated using various spectroscopic analyses.