Nonlinear optical (NLO) susceptibility of Ethidium Bromide (EB) dye, an excellent NLO material, dissolved in different solvents, including ethanol, methanol, acetone, 1-propanol, DMF, and DMSO is reported. Z-scan tool is used to measure the NLO features of EB dye using open and closed aperture methods. The peak-valley transmittance graph indicating self-defocusing nonlinearity in the nonlinear refractive index of EB dye in different solvents. The nonlinear absorption coefficient showed both positive and negative nonlinearity is the result of saturable and reverse saturable absorption behavior from the open aperture curve. The absorption coefficient and nonlinear refractive index of EB dye in various solvents are found to be the order of 10