This study evaluated the pretreatment effect of ethanolic extract of Terminalia chebula fruit (TCE) on LPS-induced acute lung injury in mice. TCE doses of 100 and 200 mg/kg bwt were administered for seven days before LPS exposure. Pretreatment effects were assessed by measuring lung oedema, total protein, MPO, ferritin, D-dimer, oxidative stress, and histopathology, along with iNOS, and MMP-9 expression. TCE pretreatment reduced lung edema, total protein, MPO, ferritin, and D-dimer levels significantly. It also decreased MDA content while enhancing antioxidant enzymes catalase and glutathione. Histopathology showed moderate lung injury reduction at 200 mg/kg bwt TCE, with less neutrophilic infiltration and better lung tissue architecture. Furthermore, the administration of TCE significantly reduced the expression of iNOS and MMP-9. Thus, TCE pretreatment alleviates LPS-induced acute lung injury by reducing inflammatory markers and oxidative stress.