Diagnosis of joint infections is often challenging due to low specimen volumes, low sensitivity of Gram stains and long incubation times of cultures. Digital PCR (dPCR) is a molecular tool that can detect nucleic acid targets with high sensitivity and resistance to inhibition. A 3 hour dPCR assay targeting the 16S gene was performed on archived synovial fluids. The assay detected the 16S gene directly from 4 µL of joint fluid without nucleic acid extraction. In 43 culture positive neat synovial fluids, the dPCR instrument detected 31 (72%) as positive, and 12 (28%) as indeterminate. In 49 culture negative specimens, dPCR was negative for 34 (69%), indeterminate for 14 (29%). The detection of bacteria was similar to real-time PCR performed on extracted specimens and demonstrated superior sensitivity to Gram stain. This technique shows potential as a rapid detection method for bacterial pathogens in synovial fluids, with optimization to improve specificity.