Foot-and-mouth disease (FMD) is endemic in Pakistan and serotypes O, A and Asia-1 of FMD virus (FMDV) are responsible for the outbreaks in the country. The dominant serotype is type O represented by the O/ME-SA/PanAsia-2 lineage, which has diverged into different sublineages. Characterizing circulating viruses helps to trace the origin of outbreaks and provides evidence to select appropriate vaccines. The present study characterized viruses belonging to the O/ME-SA/PanAsia-2 lineage collected from bovines during 2012-2021. Phylogenetic analyses using the VP1 coding sequences revealed that these viruses grouped into five sublineages, of which two have been described previously, i.e. O/ME-SA/PanAsia-2