The photocatalysis performance of biochar (BC) was often underestimate when it was employed to remediate antibiotic-contaminated soil. In our study, we adopted sawdust, rice husk, and tobacco straw to prepare biochar at three carbonization temperature (300 °C, 500 °C, and 700 °C) and explored the effect of biochar amendment on the sunlight photolysis of typical antibiotics tetracycline (TC), sulfamerazine (SM1), and enrofloxacin (ENR) on soil surfaces. The photolysis of three examined antibiotics in the surface layer of soil-biochar followed a quasi-first-order reaction kinetics model (R