Eight previously undescribed resin glycosides, namely calonyctins K-R (1-8), and nine known resin glycosides (9-17), were isolated from Ipomoea muricata via the UPLC-TOF-MS/MDF guided isolation approach. The isolated resin glycosides included 25-, 27-, and 19-membered ring resin glycosides, as well as resin glycosidic acids. Notably, resin glycosides calonyctins G and H (10 and 11) exhibited considerable cytotoxicity against the A549 and HCT-116 cell lines, with calonyctin H (11) showing IC