The proposed study uses a special procedure to verify that an analyte assessment operation is sustainable and environmentally friendly. When the targeted analyte molecule (alogliptin drug, ALT) lacks chromophoric or fluorogenic groups, it cannot be analyzed without marking or structural amendment. The obeyed phenomenon (marking process) could be achieved via fluorescence probing or marking using tagging agents. An innovative reagent pair consisting of trioxosulfate ion and o-Phthaldialdehyde (OPD) is used in the described analytical approach. This reagent is used to build a novel fluorescent nucleus and can be detected using the developed fluorimetric approach. ALT's amino moiety could condense trioxosulfate/OPD. This study presents a new, facile, green, sustainable fluorometric assay of the ALT drug. The product's response is linear at 50.00-800.00 ng mL