Elastin-like polypeptides (ELPs) are biopolymers with repetitive amino acid sequences and are known for their biocompatibility and inverse transition cycling (ITC) properties
thus, they are ideal for biomedical applications. Owing to their low yield and tedious purification process with multiple rounds of ITC, no acceptable scaled-up production process has been developed. Here, for the first time, an efficient, low-cost process for the preparation of recombinant human elastin-like polypeptide (rhELP) is reported. This process leverages high-cell-density fermentation and hollow fibre membrane (HFM) filtration technology. First, we constructed an engineered strain of Escherichia coli (E. coli) with high expression of the rhELP protein, and a yield of 0.99 ± 0.03g/L was achieved in shaker flasks by optimizing the induction temperature, induction OD