Aims: This study investigated the cancer preventive effect of black garlic on hepatocellular carcinoma. Methods: Athymicnude mice drank daily the of black garlic solution by the doses of 19 or 10 g/kg body weight in 1 month. Then, the nude mice were inoculated 106 human hepatocellular carcinoma cells. Results: The results showed that with dose of 10 g/kg daily of black garlic solution were significantly reduced in the rate of nude mice bearing tumor development and volume of tumors in nude mice. Moreover, by this dose, the black garlic also inhibited significantly the tumor grow and prolong alive time of nude mice bearing tumor in comparison to control group (p0.05). Conclusions: Drinking the black garlic shown its effect to prevent liver cancer development in vivo.