Circulating monocytes (Mo) are precursors to a subset of gastric resident-muscularis macrophages. Changes in muscularis-macrophages (MMs) result in delayed gastric emptying (DGE) in diabetic gastroparesis. However, the dynamics of Mo in the development of DGE in an animal model are unknown. Using CyTOF and computational approaches, we show a high heterogeneity within the Mo-population. In DGE mice, via unbiased clustering, we identified two reduced Mo clusters which exhibit migratory phenotype (Ly6C