There are many factors that affect on in vitro propagation in which carbonhydrate plays an important role for the growth and development of explants. In this study, callus derived from single cells of Phaleanopsis amabilis were cultured on somatic embryogenesis medium. Effect of various concentrations (10, 20, 30, and 40 g/l) of different sugars (D-glucose, D-fructose, experimental sucrose and commercial sucrose) was investigated for somatic embryogenesis. The results showed that D-glucose and D-fructose had negative effects on somatic embryogenesis of Phaleanopsis amabilis after 7 weeks culture. Especially, D-fructose caused necrosis of all explants. Experimental sucrose and commercial sucrose seemed to be suitable for somatic embryogenesis culture of Phaleanopsis amabilis. The highest somatic embryogenesis rate, healthy globular and homogeneous embryo with highest fresh weight (332 mg/explants) was obtained on the medium supplemented with commercial sucrose at the concerntration of 20 g/l. At concentrations of 30 and 40 g/l, all sugar types investigated in this study were not suitable for embryogenesis from callus derived from single cells.