A feeding trial was conducted to evaluate the effect of protein hydrolysates from tuna head in the diet on the survival and growth of white leg shrimp (litopenaeus vanname). Trial was conducted on five diets: one control diet (DC) containing tuna head meal as the principal protein source, three diets formulated by replacing 50 percent tuna head meal by products obtained from hydrolysis of tuna head, and one cpmmercial diet (TM) used as a reference. All five diets contained 40 percent crude protein. Three diets supplemented with tuna head hydrolysates included a diet containing the soluble protein powder (BPT), a diet containing insoluble protein powder (BPKT) and a diet containing a mixture of both soluble and insoluble protein powers (HH). Mer 6 weeks of experiment, the survival rate, weight gain, feed conversion ratio and protein efficiency ratio were compared between the different groups of shrimp. The study results showed that the survival rate of five groups of white leg shrimps (litopenaeus vanname) ranged from 83.3 to 97.8 percent. The shrimp fed diet containing soluble protein powder had the highest weight gain (140.63 percent), while the shrimp fed diet containing mixture of both soluble and insoluble protein powers had the lowest weight gain (109.06 percent). Feed conversion ratio of shrimp ranged from 1.72 to 2.12 percent. Protein efficiency ratio ranged from 1.20 to 1.48 percent. The diet containing protein hydrolysate from tuna head (soluble protein powder) improved significantly the feed conversion ratio and protein efficiency ratio.