Biểu hiện, tinh sạch và kiểm tra độc tố protein tái tổ hợp staphylococcal enterotoxin b (seb)

• Tác giả: Thị Thu Trang Hầu, Ngọc Minh Nghiêm, Thái Sơn Nguyễn, Thị Hoài Thu Nguyễn
• Ngôn ngữ: vie
• Ký hiệu phân loại: 616.94 *Bacterial blood diseases
• Thông tin xuất bản: Công nghệ sinh học, 2013
• Mô tả vật lý: 565-570
• Bộ sưu tập: Metadata
• ID: 556515

Tóm tắt

 Staphylococcal enterotoxin B (SEB) of the bacterium Staphylococcus aureus is one of the most dangerous causes of food poisoning. In this study, the authors expressed two kinds of SEB antigens: SEB1 is native protein
  SEB2 is a mutant with some amino acid substitutions with histidine to tyrosine at four codon 12
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  105 and 121 for 5 hours at 30°C with 0,1 mM IPTG. The recombinant SEB proteins were successfully purified by Nickel Resin column. Protein concentrations of SEB1 and SEB2 after elution from the column were 0.815 mg/ml and 1.15 mg/ml respectively. The assessment of toxicity of recombinant proteins showed that SEB2 did not induce a death in mouse at the dose of 10xLD50 while native SEB caused the death with 60 percent at the dose of 1 xLD50 result in 100 percent death at the dose of at 3xLD50. These results are vital criteria for creating Kit for rapid detection of food poisoning caused by S. aureus.