Six compounds 12-hydroxyevodol (I), rutaevine (2), lupeol (3), tTiedelan-3-one (4), stigmasterol (5), and daucosterol (6) were isolated from the methanol extract of the leaves of Tetradium glabrifolium by various chromatographic experiments. Their chemical structures were elucidated by ESI-MS, 1D and 2D NMR experiments in comparison with the reported data.