The pathogenic fungi attacking tea cause heavy losses to the tea industry in Thai Nguyen. Among actinomycetes strains studied, the strains HT17.8 which showed the highest activity against all four strains of pathogenic fungi attacking tea (N1, D1, PL3, TB4) was chosen for this study. This new strain is capable of producing antimicrobial agents, which active in vitro against Gram positive bacteria (Bacillus subtilis) and Gram negative bacteria (Escherichia coli), fungi (Fusarium oxysporum, Fusarium solani). Obtained results indicated the strain HT17.8 has spiral spore chains, smooth spore surface. Well growth was recorded at a temperature range of 25 to 35°C and at pH range of 6 to 8, tolerated 9 percent NaCl. The strain HT17.8 showed moderate activity some enzymes as amylase, protease, cellulase. On the basis of morphological, culture, biochemical and physiological characteristics and the sequence analysis of 16S rRNA gene, the strain HT17.8 was identified as Streptomyces sp. HT17.8. This sequence was submitted on Genbank with number accession JQ012795. Optimal culture conditions for the growth and antibiotic production of the strain Streptomyces sp. HT17.8 were determined on A-4H medium.