Sacbrood disease on honey bee is caused by Morator aetatulae virus (SBV). This disease causes heavy loss on both exotic bee (Apis mellifera) and indigenous bee (A. cerana), which are being raised and exploited honey in Vietnam. Using biological measure for disease control is only able to limit the transmissive infection of this disease. Ribonuclease enzyme extracted from ox's pancreas can be considered as a new pro-biotic for prophylaxis and therapy of this disease. In this study, 1000 ml of ribonuclease enzyme solution (36unit/ml) were used for experimental treatment of sacbrood disease on the exotic bee (A. mellifera) raising in Dien Bien province. 70 SBV infected colonies were divided into 7 groups: 6 experimental groups and 1 control group (10 hives per group). 6 experimental groups were treated by two methods, such as: feeding bees and spraying on bees with the mixture solution of ribonuclease enzyme and sugar syrup. For each method, 3 different formulae of different enzyme concentrations were applied. As a result, the infected bee hives were fed with the enzyme-sugar solution with ratio: 1:100 (100ml per bee comb per time, 5 days per time, 3 times per treatment) were recovered from the disease after 60 days, while those were sprayed with the mixture solution with ratio: 1:5 and 1:9 (5 days per time, 3 times per treatment) recovered from the disease after 30 days. The honey yield from the treated bee colonies was 34-42 percent higher than that of the control colony, also the strength of the treated colonies were 2.4-3.4 combs per colony higher than that of the control colony. This experimental result can be applied for treatment of the sacbrood disease on the exotic bees (A. mellifera) as well as on the indigenous bees (A. cerana) which are seriously infected with SBV on large scale.