New Insights into Expanding the Insecticidal Spectrum of dsRNA Mediated by the High Sequence Identity between dsRNA and Nontarget mRNA.

• Tác giả: Jiasheng Chen, Haochen Du, Nan Jiang, Sengodan Karthi, Mao-Ye Li, Su Liu, Cheng-Wang Sheng, Jiayu Tan, Chenyu Zhang, Kezhi Zhao
• Ngôn ngữ: eng
• Ký hiệu phân loại: 636.0885 Animal husbandry
• Thông tin xuất bản: United States : Journal of agricultural and food chemistry , 2025
• Bộ sưu tập: NCBI
• ID: 55739

Tóm tắt

 RNA interference (RNAi) is being used to develop methods to control pests, yet its widespread application is limited by the high comprehensive application cost of dsRNAs. Here, we utilized the high identity matching between double-stranded RNA (dsRNA) and nontarget genes to achieve expanding the dsRNA insecticidal spectrum. First, we found that dsRNA was more likely to induce off-target effects in genes with higher transcript levels and higher sequence identity
  the existence of either a completely contiguous matching sequence exceeding 15 nt or a partially contiguous matching sequence of 24 nt between genes can lead to off-target effects in