Định lượng felodipin trong huyết tương người bằng phương pháp sắc ký lỏng siêu hiệu năng ghép nối với detector khối phổ

• Tác giả: Cao Sơn Đoàn, Thanh Hải Nguyễn, Thanh Huyền Phạm, Mạnh Hùng Tạ, Thị Thanh Huyền Vũ
• Ngôn ngữ: vie
• Ký hiệu phân loại: 544.92 Qualitative analysis
• Thông tin xuất bản: Dược học, 2014
• Mô tả vật lý: 39-44
• Bộ sưu tập: Metadata
• ID: 557405

Tóm tắt

A highly specific method using ultra performance liquid chromatography tandem mass spectrometry was developed for determination of felodipine in human plasma. Felodipine and the internal standard were extracted from human plasma with diethylether and chloroform. The analytes were chromatographed on a C18 column (50 x 2.0 mm, i.d., 1.8 pm) with isocratic elution at a flow rate of 0.2 ml/min using 2 mM ammonium acetate buffer-methanol-acetonitrile and isopropanol in suitable ratio. The Thermo TSQ UPLC-MS/MS was operated under the selected reaction-monitoring mode using the electrospray ionization technique. The method was validated over the concentration range of 0.2 to 20 ng/ml for felodipine. The intra- and inter-day precision and accuracy were within 90 percent - 110.0 percent. The recovery was 84.3 - 86.8 percent for felodipine and 92.9 percent for the internal standard. Total run time was just 2.0 min. This method proved practically applicable to BA-BE studies of felodipine preparations.