Four compounds: myristyl myristate (1), hispidulin (2), eupafolin (3) and tagitinin A (4) were isolated from the dichlomethane leaf extract of Tithonia diversifolia (Hemsl.) A. Gray. Their structures were elucidated by analysis of IR, MS, and NMR spectra and comparison with the published data. Compounds 1 and 3 were isolated for the time from this plant.