Maize leaf and sheath spot disease caused by Epicoccum sorghinum is an emerging disease of maize in China. To disentangle the molecular pathogenesis, we sequenced the genome and infection transcriptomes of the E. sorghinum strain NJC07. The genome was sequenced on Oxford Nanopore GridION and Illumina NovaSeq 6000, producing a near-complete gapless nuclear genome assembly of 32.69 Mb at 285.20-fold depth, comprising 23 contigs (including 12 full-length chromosomes) with an N