The aim of this study is to assess the knowledge of food hygiene and safety of the people in Chuc Son in 2009. Methods: This is a cross-sectional study of 384 housewives in Chuc Son. Results: The percentage of people who knew about the transmission of diarrhea by people infected with cholera to healthy people, or from sick human wastes through flies-to-food-to-people was high with 89 percent and 90.4 percent, respectively. 38.0 percent percent of people did not know about disease transmission through patient-to-waste-water-food route to people. Most people thought that shrimp sauce and dog meat accounted for 90.4 percent of pathogens. Many people also blamed salad leaves (77.3 percent) and blood (25.8 percent) for some infectious diseases. Knowledge of sanitation practices was high with 97.4 percent of the respondents threw household wastes to garbage truck, 2.6 percent of the people burned house wastes in their gardens. As for household sanitation accesses, 92.7 percent of the households had general drainage sewer system while only 7.3 percent has drainage line ponds. Conclusions: In general, the majority of people of Chuc Son knew the mode and sources of diarrhea. They also demonstrated excellent knowledge about sanitation practices to prevent pathogen infection causing diarrhea.