In this study, the extration of black sesame oil using supercritical fluid extraction was studied. Extraction conditions strongly influences on the extraction yield with positive relationship. When the temperature, pressure and flow rate of CO2 increased, the extraction yield increased
the decrease of raw material particle size shortened extraction time. From the investigation, the optimal conditions were selected: 60°C, 400 bar, 45 g/minute of CO2, 315-800 um particle size and extraction time of 180 minutes. The extraction yield using SFE in 3 hours was equal to the extraction yield using petroleum ether in 8 hours. The black sesame oil extracted in SFE had a better quality compared with that of organic solvent extraction, which expressed as higher iodine, lower acid and saponification indices and higher unsaturated fatty acid content.