Race Nr.SL.o3 (Nomuraea rileyi) isolated from Spodoptera Btura and good developed on MYAR medium, after 25 days of incubation, with average temperature from 26.7 to 27.5°C and average humidity 72.5-79.0 percent, reaching colonies diameter was 77.5 mm and the number of spores formed after 15 days was from 5.8 to 6.5 x 10 exponent 8 CFU/ml. Conidiaphore have a forme of banana bunch or like flowers, developed from hyphae, straight or curved, in the crystal, with a total length of 75 - 167 x 2.5 - 3.5 um. Each stem has 3 bunch banana bunch, horizontal size 18.5 - 22.5 x 8.5 - 10 um, distance from beam 1 to 2 is 18.5 - 23 um. Conidia: unicellular colored white, glossy surface, ellipse, size 5-6 x 2- 3.5 micrometers. Race Nr.SL.03 (Nomuraea rileyi) was laboratorial tested on Spodoptera litura at 2nd stage at a concentrafion of 3 x 10 exponent 8 CFU/ml: After 13 days of treatment, there were 72.5 percent to 75.0 larvaes got death, among them 25-35 percent got fungal mycelia and conidies grown. After 12 days of treatment with Spodoptera litura on onions, result showed that larvaes got death at 70.5 percent and 50 percent of them got fungal mycelia and conidies grown.