The nucleoprotein gen N of 46 confirmed measles isolates in Hanoi, Thanh Hoa, Bac giang, Hoa binh, Nghe an, Ha giang, Lai chau, Lao cai between 2006-2013 were analyzed by PCR-RFLP and sequencing. PCR-RFLP analysis revealed the circulating of genotype H1 in collected isolates. The sequence of 660 bp specific DNA of measles virus confirmed the dominant of genotype H1 in Vietnam, 2006-2013. Thus, PCR-RFLP method can be used for molecular epidemiology analysis of measles vivus.