The authors are reporling a case of a 42-year-old male from Hanoi, who came to see a doctor complaining of acute onset of nodule penis. A diagnosis of nodule on the wall of penis was made and an electrosurgery was canied out. The nodule was oval shape with 2 cm length. On opening the tumor, lancet-shaped worm were seen emerging from it with leaft shape, light yellow color and 1 mm diameter. The worms were identified as Clonorchis sinensis by Department of Parasitology and Department of Molercular, in National Institute of Malariology, Parasitology and Entomology. Sequence data were analyzed the similarity compared with Clonorchis sinensis from Nam Dinh - Vietnam and Guang Dong - China were 99.9 percent and 98.9 percent. The authors reported here the first case infected with Clonorchis sinensis as nodule on the pensis in Vietnam confirmed by molecular analysis. After the operation the patient was treated with praziquantel and he had an uneventful recovery.