This study was performed to establish the protocols of isolation, in vitro culture and cryopreservation of Bovine Oviduct Epithelial Cells (BOECs) used for bovine embryo technology. Bovine oviducts were collected from slaughterhouse and kept in Phosphate Buffered Saline (PBS) medium provided with Penicilline 100.000 UI/Liter and Streptomycine 0.05 gr/Liter, pH 7.3-7.4 at 37°C. They were transferred to laboratory within 06 hours. BOECs were isolated by modified procedure of Walter (1995). They were then cultured in Dulbecco's Modified Eagle Medium (D'MEM), 10 percent Fetal Bovine Serum, 5 percent CO2, 37°C (modified procedure of Kim et al. (2011) and McGowan (2011)). The results showed that 71-88 percent BOECs' pieces continued to develop in the above condition. After 48 hrs of in vitro culture, BOECs were ITozen in D'MEM supplemented by 10 percent Fetal Bovine Serum and 10 percent Dimethylsulfoxide (modified procedure of Fazekasova et al. (2010)). Twenty eight BOECs' lines were cryopreserved in liquid Nitrogen (-196°C). After thawing at 37°C during 01 minute, BOECs could develop in culture's condition (D'MEM, 10 percent Fetal Bovine Serum, 5 percent CO2, 37°C) in vitro. In conclusion, the protocols of isolation, in vitro culture and cryopreservation of Bovine Oviduct Epithelial Cells (BOECs) were successfully established in the laboratory.