The purpose of this study was to discover the optimal time to harvest F1 Savior tomatoes in order to maintain post-harvest freshness. Tomatoes were collected in Bac Ninh at 6 stages of ripening: mature green, breaking, turning, pink, light red and dark red. In a laboratory were evaluated by analysis of physiological and biochemical indicators. The results of this study showed that mature turning and mature pink tomatoes can be stored for the longest period of time. To prove this result, the authors keep harvested tomatoes at 4°C and at 27°C. The results indicate that the storage time of maturity 3 and maturity 4 is 20 days at 27°C and 25 days at 4°C.