Enzyme application has emerged as a powerful tool for providing benefits to paper mills in various sections like pulping, bleaching, refining or beating and deinking. In this paper, the laboratory study on enzymatic treatment of mixed hardwood (acacia CTMP and LBKP 90) and softwood pulp (NBKP 90) has performed for evaluating the effect of commercial enzyme FibreZymeTMLBR by applying the treatment at different temperature (from 30°C to 60°C) reaction times (from 30 min to 180 min) and enzyme dosage (from 215 U/kg to 720 U/kg) that were comparable with non-ernzyme treated samples before the refining step. The valid technological regime was chosen as follows: enzyme dosage of 420-440 U/OD kg pulp, treatment temperature of 40-45°C and treatment time of 60-120 min. This treatment improved pulp rerming and quality by development of fibrillation and also affected physical strength properties of paper. There was a significant decrease in refining time to achieve same level of Schopper Riegle Freeness (OSR) of pulp. The fiber morphology difference between before and after treatment was revealed by the microscopic observations performed by Scanning Electron Microscope (SEM). The SEM analysis showed that the surface of cellulase treated fibre had some swelling and fibrillar phenomenon that lead to strong paper properties such as tear index, tensile index and bursch index.