From the methanol extracted from powdered stem bark of Dillenia indica Linn. (collected in Tuyen Quang province) were isolated three compounds: Beta-sitosterol 1,betulin aldehyde 2 and betulinic acid 3. Their structures were elucidated using the IR, 1H-NMR, 13C-NMR and MS spectral methods. The biological activity of two compounds (2 and 3) was identified in in vitro condition.