9-Methylenedioxophenantridine (trisphaeridine, 1), 5,6-dihydrobicolorine (2), 3-epimacronine and O-methylpretazettine (3) O-methylpretazettine (4) were continuously isolated from the whole plant of Hymenocallis littoralis. Among them O-methylpretazettine was found for the first time from the species. Structures of the compounds were elucidated by means of spectroscopic methods.