The goal of this study is to isolate and culture dental pulp stem cells (DPSCs) from mouse mandibular incisors. Mandibular incisors from 2 - 3 weeks old mice were collected and sterilized in a solution of 400 IU/ml Penicilline and 400 IU Streptomycin for 20 minutes. Dental pulp fragments were isolated and incubated in 35mm-dish containing DMEMF12, supplemente:d with 10 percent FBS, 100UI/ml Peniciline, 100ug/ml Streptomycine, at 37°C and 5 percent CO2. After 3 weeks, confluent cells were stripped and transferred to 25 cm2 tissue culture flask and maintained up to passage 4 (P4). At P4, DPSCs were identified using immunofluorescent flow cytometry method. Dental pulp culture cells at passage 4 expressed high levels of mesenchymal stem cell markers such as CD73, CD90, CD105, and a low level of endothelial hematopoietic cells such as CD19, CD34, and CD45. the authors also found that DPSCs reach a maximal proliferation levels at day 7, and have a cell population doubling time of 45 hours. Conclusion: the authors have successfully isolated, cultured and characterized dental pulp stem cells in mice using a simple tissue cell culture method.