Kiwi (Actinidia deliciosa) is known as a kind of medicinal plant, as well as fruit with high content of vitamins and minerals. Currently, Kiwi has been grown and produced in many countries around the world
however, it has not been grown popularly in Vietnam. In order to create the initial source for the propagation, in this study, the authors have successfully regenerated Kiwi via organogenesis from in vitro leaf culture. After 6 weeks, the authors found that leaf samples were cultured on MS medium supplemented with 30 g/l sucrose, 8 g/l agar, 0.02 mg/l NAA and 0.5 mg/l TDZ offered the highest number of shoots (13.33 shoots/sample), callus induction from leaf sample can be observed only on MS medium supplemented with kinetin and shoot formation on MS medium with high concentration of BA. The highest shoot formation number was achieved when they were subcultured on MS medium culture supplemented with 30 g/l sucrose, 8 g/l agar and 0.01 mg/l TDZ (8.33 shoots/sample after 6 weeks of culture). The shoots then were rooted on MS medium supplemented with 30 g/l sucrose, 8 g/l agar and 0.05 mg/l NAA. The Kiwi plantlets were able to survive well when grown in the system covered with nylon (98.5 percent), to grow and to develop well in the nursery.