Gram-negative bacteria resistance to carbapenems due to the expression of the New Delhi metallo-beta-Iactamase 1 (NDM-1) gene are one of the major global health problems. Previously, NDM-1 positive bacteria have been reported in bacteria isolated from both clinical and environmental samples in Vietnam. To investigate whether NDM-1 positive bacteria contamination exits in the Viet Duc Hospital, the authors collected 200 samples from 8 major sites often directly in contact with patients such as the nurse hands, medical trolleys, floor surfaces, patient bed sheets, toilet covers, and medical equipment, plus other minor sites such office table tops and telephone in three surgical departments (urology, gastro-enterology and hepato-biliary surgery). Samples were transported to the microbiology laboratory and tested for carabapenem resistance and the presence of the NDM-1 gene by PCR. NDM-1 positive bacteria were further identified by biochemical testing (API-20E strip, Biomerieux) and susceptibility testing. The results shown: 5/200 (2.5 percent) samples positive with the NDM-1 gene, three samples were patient qed sheet, one was from cover of medical bin-wash disposal, and one in toilet cover in Urology and Hepatobiliary Surgery departments. All of positive NDM-1 were extensively drug resistant, but remained susceptible to colistin.