According to the World Heart Federation, Cardiovascular diseases are the world's largest killers, claiming 17.5 million lives a year. Acute coronary syndrome (ACS) is a health issue of great concern worldwide due to its sudden attack, high mortality rate and serious sequelae, with the number of patients increasing rapidly in both developing and developed countries. Recent studies have discovered genetic association between polymorphic variants in candidate genes and atherosclerosis. The matrix metalloproteinase (MMP) family is a potential candidate gene systems for ACS, of which MMP-9 is the most attractive target research due to its vital role in extracellular matrix degradation and atherosclerotic plaque reproduction. In this study, sequencing of MMP-9 gene from forty-one inviduals (9 normal samples, 14 unstable angina and 18 acute myocardial infarction patients were Vietnamese carried out. By comparing MMP9 gene sequence between ACS group and the control without ACS, the -1562CT polymorphisms were detected in MMP9 genes of 3 unstable angina patients and 7 acute myocardial infarction patients. The results show the first data of MMP-9 gene sequence from Vietnamese ACS patients.