Purpose: To describe clinical and microbial characteristics of bacterial conjunctivitis in children and evaluate the result of treatment with Levojloxacin 0.5 percent. Subjects and methods: Clinical interventional prospective study on 30 children under 16 years of age examined at VNIO from May to August 2013. Results: The mean age was 3.91 + or - 4.46,60 percent male, 40 percent female, 93.3 percent occurred both eyes. Time before being examined at VNIO was 7.57 + or - 4.35 days. 100 percent had red eye, conjunctival discharge. 56.7 percent had self treatment, 40 percent was treated at local eye centres, 62.1 percent with antibiotics, 13.8 percent with antibiotics combined steroid 10 percent had contagious source. 6.7 percent had upper respiratory injlammation. 100 percent had mucopurulent discharge and palpebral conjunctival injection. 10 percent had corneal defects. 76.7 percent was in moderate and severe condition. Gram-positive cocci in 50 percent. No positive bacteriologic cultures. 96.7 percent reduced clinical signs after 3 days, 70 percent cured after 7 days and 100 percent cured after 14 days of treatment with levojloxacin 0.5 percent. Mean time for cure was 7.33 + or - 3.15 days. No side effects were detected. Conclusion: 100 percent bacterial conjunctivitis in children had red eye, conjunctival discharge, mucopurulent discharge and palpebral conjunctival injection. Most cases had self-treatment and in moderate and severe condition. Gram-positive cocci have the highest percentage. Treatment with levojloxacin 0.5 percent was effective and safe.