In vitro fertilization (IVF) is a major technique producing in vitro embryos for biomedical researches and biodiversity conservation in pigs. Main materials in this case are' sperm frozen thawed and oocytes. In this study, the authors evaluated effect of frozen semen on the ability to form male pronuclei and subsequent development of porcine oocytes matured and fertilized in vitro. Sperm freezing and IVF was carried out according to the method of Kikuchi et al., 2002. The parameters for the evaluationare the incidence of male pronuclei formation, monospermy, cleavage and the morula-blastocyst rates. Frozen sperm from two Landrace porcine TL1 and TL2 stored in nitrogen liquid is used. There was no difference (P0,05) in male pronuclei formation rate and monospermy rate between TL1 and TL2 groups(85,7 + or - 14,3 and 57,03 + or - 6,83, respectively), (53,48 + or - 5,52 and 39,61 + or - 3,7, respectively). The cleavage, morula/blastocyst rates did not differ between TL1 and TL2 (51,49 + or - 5,77 and 29,96 + or - 7,26, respectively) and (52,99 + or - 8,13 and 16,69 + or - 4,31, respectively). The results show that the frozen semens are available for using in pig IVF system.