The genus Gymnosporia (Celastraceae) consists of about 300 species in the world. The plants of this genus are rich in triterpenoid, sesquiterpenoid, alkaloid, flavonoid, and phenolic that exhibited a variety of biological and phannacological activities. The EtOAc extracts of the stems of Gymnosporia sty/osa showed cytotoxic activity against cancer human cell line KB (94 percent inhibition at ug/mL). In this paper, the authors report the isolation and structural elucidation of 8 compounds abruslactone A (1), beta-amyrin (2), canophyllol (3), 28,29-dihydroxyfriedelan-3-one (4), friedelin (5), syringaldehyde (6), syringic acid (7), and 3,4-dihydroxybenzoic acid (8) ITom the stems of this plant. It is the first time this plant had researched about chemical constituents in Vietnam as well as in the World.