Optimization of the fluorescence in situ hybridization technique for prenatal diagnosis some of chromosome aneuploidy. Background: Fluorescence in situ hybridization (FISH) is cytogenetic molecular technique, with used DNA probe 13, 21, 18, X, Y, FISH is the rapid detection technique of aneuploidy chromosome. Objectives: (1) Optimization some of step preparation slide before and after hybridization
(2) Application of the FISH technique for prenatal diagnosis some of aneuploidy chromosome. Methods: In this study, the authors treated 12 amniotic fluid samples after that, the authors applied DNA probe to detect some of aneuploidy chromosome. Results: 1 sample was Down syndrome trisomy 21
1 sample was mosaic (65 percent normal
35 percent trisomy 21)
1 sample was monosomy X
1 sample trisomy 18
8 samples were normal. Conclusions: (1) the authors optimized and reduced some of chemical in preparation slide. (2) The FISH analysis result of 11/12 samples were compared to the chromosome analysis result. 1 sample wasn't analized chromosome.