Introduction: Urinary tract infection (UTI) represents the third cause of infection in children. The antibiotic resistance has become an increasingly pressing clinical issue in many countries. Objectives: The aim of the study was to assess the susceptibility of urinary pathogens to commonly used antibiotics and the cause of UTI in children admitted in Pediatrics Department of Hanoi French Hospital from February 2007 to December 2010. Materials and Methods: Inclusion criteria were clinical symptoms and significant bacteriuria (at least 100.000 colony forming units/ml urine). The antibiograms were made by Kirby - Bauer methodology at Hanoi French hospital microbiology laboratory. Results: A total of 66 UTI patients in this study. The most common causative agents were Enterobacteriacea (91 percent). In which, the majority isolates were Escherichia Coli (62.2 percent)
followed by Enterobacter (16.7 percent). The results of the antibiograms showed that E.coli was high percent of resistance to Ampicilline (76.7 percent) and Cotrimoxazole (73 percent). The lowest resistance was identified to Augmentin, Cefotaxim, Cefriaxone, Ceftazidim and Amikacine. Conclusion: Ampicillin and Cotrimoxazole should be avoide for the initial use as the empiric treatment of UTI. The high resistance of urinary pathogens to commonly used antibiotics required further comprehensive observation.