Duchenne Muscular Dystrophy (DMD) caused by dystrophin gene mutation, is:responsible for 60 - 65 percent of DMD patients. About one third of DMD patients are carriers of the new mutation and the others inherit the abnormal gene from the heterozygous mother. Multiplex Ligation - dependent Probe Amplification (MLPA) has been commonly used to detect mutation in inhsrited diseases because MLPA is a rapid and an accurate technique. In this study, MLPA technique was used to detect the rate of female carriers in family with DMD. The results showed that 36/68 of female members have been detected as carriers, accounted for 55.8 percent.