Bacillus subtillis derivedgeneCspB, coding for RNA chaperone functional protein enables mRNA stable as single-stranded in adverse conditions. Therefore, the expression CspB protein in transgenic plant could increaseresistant ability of plants to environmental stresses such as cold, heat and drought In order to optimize the expression of the bacterial gene in plants, an artificial- synthesis CspB(modiCspB) gene with 46 nucleotide modifications from AfT to G/C was obtained to achieve a more compatible expression g~ne in plant. ModiCspB gene was inserted in pCAMBIA1300 under the control of 35S promoter and transformed in to immature embryos of three maize inbred lines of Vietnam V152N, C436 and C7N by Agrobacterium-mediated method. The results of transformation obtained 68 regenerated plants survived in the green house, 62.02 percent of total regenerated plants. In which, the line V152N produced the highest (70.37 percent) of transgenic plantsand the lowest was C463 47.83 percent). The transformation efficiency was 0.56 percent. after fertilityassessment and PCR analysis.. As consequence, the research has provided the initial transgenic maize plants by improvementof the stress tolerance.